Journal: Advanced materials technologies

Article Title: Perforated and Endothelialized Elastomeric Tubes for Vascular Modeling

doi: 10.1002/admt.201800741

Figure Lengend Snippet: Endothelialization of the perforated PDMS tubes. (A, B) Fluorescence micrographs showing the proliferation of HUVECs in the perforated PDMS tube during a 6-day culture period. (C) Quantification of numbers of HUVECs during the 14-day culture. Data are presented as mean ± standard deviation (STD); N = 6. (D) Cytoskeleton characterization of the endothelialized, perforated PDMS tubes at low and high magnifications. (E) Confocal orthogonal views showing the expressions of CD31 of the formed confluent endothelium.

Article Snippet: Cells HUVECs and HUVECs expressing GFP (Angio-Proteomie, Boston, MA, USA) were cultured in 75-cm 3 flasks in endothelial growth medium (EGM, Lonza, Basel, Switzerland) with 10 vol.% fetal bovine serum (FBS, Thermo Fisher, Waltham, MA, USA) and 1 vol.% penicillin-streptomycin (pen/strep, Thermo Fisher).

Techniques: Fluorescence, Standard Deviation

Journal: Advanced materials technologies

Article Title: Perforated and Endothelialized Elastomeric Tubes for Vascular Modeling

doi: 10.1002/admt.201800741

Figure Lengend Snippet: Tumor spheroid angiogenesis from perforated PDMS tubes. (A, B) Areas and circularities of the MCF-7 breast tumor spheroids at different time points of culture in the GelMA/collagen I matrix. Data are presented as mean ± standard deviation (STD); N = 16 (Day 3), 21 (Day 4), 21 (Day 5), 20 (Day 6), and 15 (Day 7). Spheroids were not formed until Day 3. (C, D) Area and circularity distributions of the MCF-7 breast tumor spheroids at Day 7. (E) Morphologies of the MCF-7 breast tumor spheroids and outward migration of the MCF-7 cells at Day 1 and Day 7. (F) VEGF secretions by the MCF-7 breast tumor spheroids at different time points of the culture, either in the absence or in the presence of the perforated, endothelialized PDMS tube. Data are presented as mean ± standard deviation (STD); N = 3. (G) Directional endothelial cell migration from the perforated PDMS tube towards the MCF-7 breast tumor spheroid at different time points of culture in the GelMA/collagen I matrix, under static conditions. HUVECs were GFP-labeled. The autofluorescence from the MCF-7 spheroid might be attributed to its high cell density.

Article Snippet: Cells HUVECs and HUVECs expressing GFP (Angio-Proteomie, Boston, MA, USA) were cultured in 75-cm 3 flasks in endothelial growth medium (EGM, Lonza, Basel, Switzerland) with 10 vol.% fetal bovine serum (FBS, Thermo Fisher, Waltham, MA, USA) and 1 vol.% penicillin-streptomycin (pen/strep, Thermo Fisher).

Techniques: Standard Deviation, Migration, Labeling

Journal: PLoS ONE

Article Title: E-Selectin Mediated Adhesion and Migration of Endothelial Colony Forming Cells Is Enhanced by SDF-1α/CXCR4

doi: 10.1371/journal.pone.0060890

Figure Lengend Snippet: A) LPS-treated HUVEC cells demonstrate significant cellular injury and increased lactate dehydrogenase (LDH) after 3h (** p<0.01). B) Gene expression of E-selectin, VCAM-1, ICAM-1 and SDF-1α induced in HUVECs after endotoxic injury was determined by RT-PCR relative to GAPDH expression (* denotes p<0.05 and ** p<0.01 in comparison with baseline). C) E-selectin levels after 12 hours in conditioned media from LPS-treated HUVEC cells (H LPS ) compared with ECFCs exposed to conditioned media from control HUVEC cells (H) or LPS-treated HUVECs cultured in the presence of anti-CD62E which inhibits soluble E-selectin (H+anti-CD62E) LPS , as measured using ELISA.(** p<0.01)

Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were obtained from the supplier (Lonza) and cultured according to instructions.

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Expressing, Comparison, Control, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: E-Selectin Mediated Adhesion and Migration of Endothelial Colony Forming Cells Is Enhanced by SDF-1α/CXCR4

doi: 10.1371/journal.pone.0060890

Figure Lengend Snippet: A) ECFC adhesion to fibronectin following exposure to conditioned media from control HUVEC cells (H), from injured HUVEC cells (H LPS ), and from LPS-treated HUVECs with the addition of anti-CD62E (H+anti-CD62E) LPS and shown as cells per high powered field (hpf) (* p<0.05). B) Adhesion of ECFCs to fibronectin following 2 hour incubation with E-selectin at 2 µg/ml (E2) or 4 µg/ml (E4) compared to controls (0 µg/ml, E0), in the presence of anti-CD44 and E-selectin (4 µg/ml) and VEGF-A 100 ng/ml (VEGFA). (* p<0.05). C) Migration of ECFCs towards E-selectin in the lower chamber at 2 µg/ml (E2) or 4 µg/ml (E4) compared with controls containing no E-selectin in the lower chamber (E0) or when ECFCs were incubated in the presence of anti-CD44 with E-selectin 4 µg/ml in the lower chamber (A+E4), and to SDF-1a (100 ng/ml) in the lower chamber (SDF). (* p<0.05). D) Photgraphs of the underside of porous membranes used in the migration assays described in part C.

Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were obtained from the supplier (Lonza) and cultured according to instructions.

Techniques: Control, Incubation, Migration

Journal: PLoS ONE

Article Title: E-Selectin Mediated Adhesion and Migration of Endothelial Colony Forming Cells Is Enhanced by SDF-1α/CXCR4

doi: 10.1371/journal.pone.0060890

Figure Lengend Snippet: A) SDF-1α mRNA expression was assessed after endotoxic injury in HUVECs with an early decrease in expression that was evident at 3 hours and persisted up to 12 hours. (* p>0.05). B) Following LPS treatment of MRC5 cells, mRNA expression of SDF-1a is increased after 6h (* p<0.05). Gene expression is reported relative to GAPDH.

Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were obtained from the supplier (Lonza) and cultured according to instructions.

Techniques: Expressing, Gene Expression

Journal: PLoS ONE

Article Title: E-Selectin Mediated Adhesion and Migration of Endothelial Colony Forming Cells Is Enhanced by SDF-1α/CXCR4

doi: 10.1371/journal.pone.0060890

Figure Lengend Snippet: A) E-selectin expression in HUVEC cells following endotoxic (diamonds), hypoxic (squares) and radiation injury (triangles). ** p<0.01 for endotoxic injury at 3h compared to 0h. B) SDF-1α expression in HUVEC cells following endotoxic (diamonds), hypoxic (squares) or radiation injury (triangles). ** p<0.01 for hypoxic injury at 6h compared to 0h. C) SDF-1α expression following endotoxic injury to MRC5 cells (triangles) and HUVEC cells (squares), ** p<0.01 when comparing expression levels at 6h. D) SDF-1α expression following hypoxic injury to MRC5 cells (triangles) and HUVEC cells (squares), ** p<0.01 comparing expression levels at 6h; * p<0.05 comparing expression levels at 6 hours in MRC5 cells to 0 hours.

Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were obtained from the supplier (Lonza) and cultured according to instructions.

Techniques: Expressing